Bread wheat callusogenesis and organogenesis using mature embryos as explants

Background. Bread wheat (Triticum aestivum L.) is one of the staple cereal crops, so it great interest to breeders and researchers requires constant monitoring existing cultivars, including development new ones through classical breeding modern gene engineering. The key stage in these techniques successful callusogenesis organogenesis intarget objects. With this view, regeneration potential two spring (‘Saratovskaya 55’ ‘Sigma’) three winter (‘Tanya’, ‘Fisht’ ‘Pamyat’) cultivars bread was assessed, optimal conditions were identified for callus induction using mature embryos. Materials methods. Immature embryos five used study. vitro morphogenetic evaluated under impact abiotic factors: preliminary exposure grains cold use exogenous hormones (2,4-D various concentrations). Pretreatment with carried out as follows: sterilized incubated on hormonal medium at a temperature 4 °C 2 weeks, then transferred 26 more weeks. efficiency rhizogenesis assessed numbers calluses, regenerated acclimatized plants calculated. Results conclusions. analysis made possible identify ‘Sigma’ their high regenerative potential. It also shown that can serve good stimulating factor producing large number but regenerants are better induced normal conditions. results depended concentration applied. Universal morphogenesis not identified.